In Vitro Ferts


 

Why Xenopus?
Purchasing
Water
Water Systems
Feeding
Lighting
The Frogs
Handling
Sexing Frogs
Testes Isolation
In Vitro Ferts
Oocyte Surgery
Colony Returns
Recycling Frogs
Natural Mating
Raising Tadpoles
Health & Disease

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Updated 1/25/2006

In Vitro Fertilization

Squeeze the eggs into a dry petri dish. Take a testis in a pair of forceps and puncture so the sperm can escape. Slowly drag the testes through the dish carefully touching each egg to ensure complete fertilization. Return the testis to its vial and place on ice. Let the eggs sit for a minute and then cover completely with 0.1xMMR. You can tell if the eggs were properly fertilized if all the eggs turn so that the animal pole is facing up.

In vitro fertilization

In vitro fertilization

 

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