You can have a natural mating to raise tadpoles or if you are
interested in expanding your colony. To do this you must inject both the male and the
female. You should inject the male with 50 u hCG and inject the non-PMSG primed female
with 750 u HCG. Put them in a 15 to 25 gallon tank together and allow
them to remain together throughout the night. You should do this at the end of
the day so the lab will be quiet throughout the night and the frogs are not
disturbed. If possible place egg crate at the bottom of the tank to keep
the frogs away from the eggs. This will prevent the eggs from being
eaten before you separate the couple.
Separate the pair the following day and leave the eggs to develop.
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